Sonic Hedgehog Protein (SHH) (AA 25-198, Cys25IleIle-Mutant)

N° du produit ABIN7314998

Protéine Recombinant Sonic Hedgehog exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Sonic Hedgehog Protein (SHH) (AA 25-198, Cys25IleIle-Mutant) (ABIN7314998)

Antigène: Sonic Hedgehog (SHH)

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Pureté: Greater than 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 25-198, Cys25IleIle-Mutant

Fonction: Recombinant Mouse SHH Protein is produced by E.coli expression system and the target gene encoding Cys25-Gly198, (Cys25Ile-Ile) is expressed.

Séquence: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20  mM PB, 150  mM NaCl, 1  mM DTT,  pH 7.4.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: SHH

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development